| Movie | 2000 | Phish: Bittersweet Motel | Trey Anastasio | |
| Movie | 2006 | Herbie Hancock: Possibilities | Self | |
| Movie | 2009 | Phish: The Clifford Ball | Himself | |
| Movie | 2004 | Trey Anastasio: Live at the Warfield | Self - guitar, vocals | |
| Movie | 2010 | Phish: Alpine Valley | Himself | |
| Movie | 2000 | Naked States | Self - Phish | |
| Movie | 1994 | Phish: 1994 Bluegrass Sessions | Himself | |
| Movie | 2013 | Phish: 2013/07/27 The Gorge Amphitheatre, George, WA | | |
| Movie | 2013 | Phish: 2013/08/31 Dick's Sporting Goods Park, Commerce City, CO | | |
| Movie | 1995 | Phish: 10-21-1995 Pershing Auditorium, Lincoln, NE | | |
| Movie | 2015 | Grateful Dead: 2015.06.27 - Santa Clara, CA | Self | |
| Movie | 2024 | PHISH: 2024-04-18 SPHERE LAS VEGAS, NV | Self | |
| Movie | 2024 | PHISH: 2024-04-20 SPHERE LAS VEGAS, NV | Self | |
| Movie | 2024 | PHISH: 2024-04-21 SPHERE LAS VEGAS, NV | Self | |
| Movie | 2024 | PHISH: 2024-04-19 SPHERE LAS VEGAS, NV | Self | |
| Movie | 2004 | Specimens of Beauty | | |
| Movie | 2010 | Phish: 10/31/2010 Boardwalk Hall, Atlantic City, NJ | Himself | |
| Movie | 2015 | Grateful Dead: 2015.06.28 - Santa Clara, CA | Self | |
| Movie | 2021 | Phish -1995-06-19 - Deer Creek Music Center, Noblesville, IN | | |
| Movie | 2015 | Grateful Dead: Fare Thee Well - 50 Years of Grateful Dead (Chicago) | Self - Performer | |
| Movie | 2010 | Phish: Coral Sky | | |
| Movie | 2001 | All Access: Front Row. Backstage. Live! | Self | |
| Movie | N/A | Phish: Live In Utica (Set 1) | | |
| Movie | 2015 | Phish - Miami, FL - 2015-01-02 | | |
| Movie | 2003 | Phish: IT | | |
| Movie | 2010 | Phish 3D | | |
| Movie | 2010 | Phish: Live In Utica | | |
| Movie | 1995 | Phish: 12-01-1995 Hershey Park Arena, Hershey, PA | | |
| Movie | 2015 | Phish - Bend, OR - 2015-07-21 | | |
| Movie | 2015 | Phish - Bend, OR - 2015-07-22 | | |
| Movie | 2011 | Phish: NYE RUN 2011 | | |
| Movie | N/A | Phish Summer 2011 Compilation | | |
| Movie | 2019 | Between Me and My Mind | Himself | |
| Movie | N/A | Phish: Live In Utica (Set 2) | | |
| Movie | N/A | Phans | Lead Guitar | |
| Movie | 2008 | Phish: Walnut Creek | | |
| Movie | 2017 | Phish: The Baker's Dozen: Night 1 - Coconut | | |
| Movie | 2002 | Phish - Live In Vegas | | |
| Movie | 2004 | Phish: Live In Brooklyn | | |
| Movie | 2009 | Phish: Hampton Coliseum | | |
| Movie | 2014 | Phish - 2014/07/03 - Saratoga Springs, NY | | |
| Movie | 2009 | Phish: Joy | | |
| Movie | 2015 | Phish - Miami, FL - 2015-01-01 | | |
| Movie | 2017 | Phish: The Baker's Dozen: Night 13 - Glazed | | |
| Movie | 2014 | Phish - 2014/07/27 - Columbia, MD | | |
| Movie | 2017 | Phish: The Baker's Dozen: Night 8 - Jimmies | | |
| Movie | 1994 | Phish: Tracking | | |
| Movie | 2014 | Phish - 2014/10/18 - Seattle, WA | | |
| Movie | 2017 | Phish: The Baker's Dozen: Night 12 - Boston Creme | | |
| Movie | 2014 | Phish - 2014/07/26 - Columbia, MD | | |
| Movie | 2012 | The Best of the Jammys, Vol. 2 | | |
| Movie | 2014 | Phish 2014/10/31 - Las Vegas, NV | | |
| Movie | 2014 | Phish - 2014/07/05 - Saratoga Springs, NY | | |
| Movie | 2010 | Music | Self | |
| Movie | 2017 | Phish: The Baker's Dozen: Night 11 - Lemon | | |
| Movie | 2017 | Phish: The Baker's Dozen: Night 9 - Maple | | |
| Movie | 2017 | Phish: The Baker's Dozen: Night 10 - Holes | | |
| Movie | 2014 | Phish 2014/11/01 - Las Vegas, NV | | |
| Movie | 2014 | Phish - 2014/07/04 - Saratoga Springs, NY | | |
| Movie | 2012 | Phish: Star Lake 98 | | |
| Movie | 2015 | Grateful Dead: 2015.07.05 - Chicago, IL | | |
| Movie | 2001 | Vermont Youth Orchestra with Trey Anastasio & Ernie Stires | | |
| Movie | N/A | Life Is a Carnival: A Musical Celebration of Robbie Robertson | Self | |
| Movie | 2010 | Phish 2010-12-31 Madison Square Garden, New York, NY | | |
| Movie | N/A | Phish 1997-11-22 Hampton Coliseum, Hampton, VA | | |
| Movie | 2014 | Phish 2014-12-31 American Airlines Arena, Miama, FL | | |
| Movie | N/A | Phish 2015-12-31 Madison Square Garden, New York, NY | | |
| Movie | 2016 | Phish 2016-12-31 Madison Square Garden, New York, NY | | |
| Movie | 2017 | Phish 2017-12-31 Madison Square Garden, New York, NY | | |
| Movie | 2018 | Phish 2018-10-31 MGM Grand Garden Arena, Las Vegas, NV | | |
| Movie | 2018 | Phish 2018-12-31 Madison Square Garden, New York, NY | | |
| Movie | 2019 | Phish 2019-12-31 Madison Square Garden, New York, NY | | |
| Movie | 2023 | Phish 2023-12-31 Madison Square Garden New York, NY | | |
| Movie | 2024 | Phish 2024-12-30 Madison Square Garden, New York, NY | | |
| Movie | 2022 | Phish 2022-12-31 Madison Square Garden, New York, NY | | |
| Movie | 2024 | Phish 2024-12-31 Madison Square Garden, New York, NY | | |
| Movie | 2022 | Phish 2022-04-22 Madison Square Garden, New York, NY | | |
| Movie | 2013 | Phish 2013-12-30 MSG | Self | |
| Movie | 2009 | Live From Bonnaroo 2009 | Self | |
| TV Show | 1992 | The Tonight Show with Jay Leno | | 2 |
| TV Show | 1993 | Late Night with Conan O'Brien | Self - Musical Guest | 1 |
| TV Show | 1975 | Austin City Limits | Self | 1 |
| TV Show | 1989 | The Simpsons | Trey Anastasio (voice) | 2 |
| TV Show | 2020 | Phish: Dinner and a Movie | Himself | 38 |
| TV Show | 1975 | Saturday Night Live | Self - Musical Guest | 3 |
| TV Show | 2025 | Everybody's Live with John Mulaney | Jerry Seinfeld (Pre-Tape Segment) | 1 |
