| Movie | 1998 | Motown 40: The Music is Forever | Self | |
| Movie | 2000 | But I'm a Cheerleader | Mike | |
| Movie | 2021 | The Bitch Who Stole Christmas | Hannah Contour | |
| Movie | 2001 | Who Is Cletis Tout? | Ginger Markum | |
| Movie | 1994 | Crooklyn | Bodega Woman | |
| Movie | 1992 | Inferno | Party Guest | |
| Movie | 2007 | Starrbooty | Starrbooty / Cupcake | |
| Movie | 2023 | Trolls Band Together | Miss Maxine (voice) | |
| Movie | 2008 | Another Gay Sequel: Gays Gone Wild! | Tyrell Tyrelle | |
| Movie | 1996 | Fled | Himself | |
| Movie | 2021 | Reno 911!: The Hunt for QAnon | Q | |
| Movie | 2023 | Nimona | Nate Knight (voice) | |
| Movie | 2024 | Ozi: Voice of the Forest | Gurd (voice) | |
| Movie | 2000 | The Truth About Jane | Jimmy | |
| Movie | 2015 | RuPaul's Drag Race: Green Screen Christmas | Self | |
| Movie | 1995 | Catwalk | Self | |
| Movie | 2005 | Disco: Spinning The Story | self | |
| Movie | 2023 | The Stroll | Self (archive footage) | |
| Movie | 2013 | Lady Gaga & the Muppets Holiday Spectacular | Self | |
| Movie | 2021 | Dragging the Classics: The Brady Bunch | | |
| Movie | 2022 | PRIDE: To Be Seen - A Soul of a Nation Presentation | Self (archive footage) | |
| Movie | 2022 | Z-O-M-B-I-E-S 3 | The Mothership (voice) | |
| Movie | 1995 | The Brady Bunch Movie | Mrs. Cummings | |
| Movie | 1994 | Nelson Sullivan's World Of Wonder | Self | |
| Movie | 1995 | Blue in the Face | Dancer | |
| Movie | 1999 | EDtv | RuPaul | |
| Movie | 1998 | An Unexpected Life | Charles | |
| Movie | 1995 | A Mother's Prayer | Deacon "Dede" | |
| Movie | 2008 | Starz Inside: Ladies or Gentlemen | Narrator | |
| Movie | 2019 | Wig | Self | |
| Movie | 1995 | Wigstock: The Movie | Self | |
| Movie | 2024 | Bob Mackie: Naked Illusion | Self | |
| Movie | 2002 | Sylvester: Mighty Real | Self | |
| Movie | 2016 | Hurricane Bianca | Weatherman | |
| Movie | 1987 | Starbooty Trilogy | Starbooty | |
| Movie | 2006 | Zombie Prom | Miss Strict | |
| Movie | 2000 | VH1 Divas 2000: A Tribute to Diana Ross | Self | |
| Movie | 1989 | Pickle Surprise | | |
| Movie | 1989 | Nelson Sullivan | | |
| Movie | 2013 | The Out List | Herself (archive footage) | |
| Movie | 2017 | Susanne Bartsch: On Top | Self | |
| Movie | 2000 | The Eyes of Tammy Faye | Narrator | |
| Movie | 2018 | Show Dogs | Persephone (voice) | |
| Movie | 2018 | Forbidden | | |
| Movie | 2018 | RuPaul's Drag Race Holi-Slay Spectacular | Self - Host | |
| Movie | 1995 | Red Ribbon Blues | Duke | |
| Movie | 2018 | The Advocate Celebrates 50 Years: A Long Road to Freedom | Self | |
| Movie | 1984 | RuPaul & Felecia Having Fun in New York - October 18, 1984 | Himself | |
| Movie | 2019 | Someone Great | Hype | |
| Movie | 1989 | Nelson Sullivan's Video Diaries | Himself | |
| Movie | 2019 | Stonewall Outloud | Narrator (voice) | |
| Movie | 2019 | The Queens | Self | |
| Movie | 1993 | RuPaul's Christmas Ball | Host | |
| Movie | 1996 | A Very Brady Sequel | Ms. Cummings | |
| Movie | 1995 | To Wong Foo, Thanks for Everything! Julie Newmar | Rachel Tensions | |
| Movie | 2019 | Trixie Mattel: Moving Parts | Self | |
| Movie | 2017 | Katy Perry: Will You Be My Witness? | Self | |
| Movie | 1993 | Fertile La Toyah Video Magazine: Inaugural Issue | | |
| Movie | 2016 | Straight Outta OZ | | |
| Movie | 1995 | The State's 43rd Annual All-Star Halloween Special | Self | |
| Movie | 2024 | Hitpig! | Polecat (voice) | |
| Movie | 2018 | BEAT. Contour. Snatched. How Drag Queens Shaped the Biggest Makeup Trends | | |
| Movie | 2020 | The Big New Year's In | Herself | |
| Movie | 2006 | Whitepaddy | Radio Kissin' | |
| Movie | 1997 | Shantay | Shantay | |
| Movie | 2021 | Workhorse Queen | Self (archival footage) | |
| Movie | 2005 | Michael Lucas' Dangerous Liaisons | Self - Non-sexual Role | |
| Movie | 2015 | Drag Becomes Him | Self (archive footage) | |
| Movie | 2002 | The Groovenians | Champagne Courvoisier | |
| Movie | 2020 | RuPaul's Drag Race: Bring Back My Ghouls | Himself (archive footage) | |
| Movie | 2025 | RuPaul’s Drag Race Live in Las Vegas 1000th Show | Self | |
| Movie | 1984 | RuPaul's Trilogy Of Terror | Sheila Jones | |
| Movie | 1999 | After Stonewall | Self | |
| Movie | 1987 | Star Booty I: The Motion Picture | Starbooty | |
| Movie | N/A | Just Between Friends | | |
| Movie | N/A | American Porn Star | Cherry Grind | |
| TV Show | 1996 | Nash Bridges | | 1 |
| TV Show | 2000 | Son of the Beach | | 1 |
| TV Show | 2018 | Follow Me | Self | 1 |
| TV Show | 1993 | Walker, Texas Ranger | Bob | 1 |
| TV Show | 2012 | Finding Your Roots | Self | 1 |
| TV Show | 2006 | Ugly Betty | Rudolph | 1 |
| TV Show | 1995 | MADtv | Self | 1 |
| TV Show | 1997 | The RuPaul Show | Himself - Host | 1 |
| TV Show | 2021 | RuPaul's Drag Race Down Under | Self - Host / Judge | 24 |
| TV Show | 1999 | Time of Your Life | | 1 |
| TV Show | 2019 | A Little Late with Lilly Singh | Self | 1 |
| TV Show | 2022 | The Tiny Chef Show | Announcer (voice) | 40 |
| TV Show | 2004 | The Soup | Self | 2 |
| TV Show | 2019 | Amphibia | Mr. X (voice) | 4 |
| TV Show | 2021 | Chicago Party Aunt | Gideon (voice) | 16 |
| TV Show | 2010 | RuPaul's Drag U | Himself | 26 |
| TV Show | 2017 | Girlboss | Lionel | 7 |
| TV Show | 2018 | The Alec Baldwin Show | Self | 1 |
| TV Show | 2009 | RuPaul's Drag Race | Self - Host | 239 |
| TV Show | 2014 | Skin Wars | Self - Judge | 29 |
| TV Show | 2020 | Drag Race Holland | Self - Video Message | 13 |
| TV Show | 1998 | Hollywood Squares | Self - Panelist | 5 |
| TV Show | 1994 | Sister, Sister | Marje | 1 |
| TV Show | 2021 | Painted with Raven | Self - Guest Judge | 2 |
| TV Show | 2013 | RuPaul Drives... | Self - Host | 33 |
| TV Show | 2014 | Last Week Tonight with John Oliver | Self | 2 |
| TV Show | 1996 | Sabrina, the Teenage Witch | Witch Judge | 1 |
| TV Show | 2021 | Drag Race Italy | Self (uncredited) | 1 |
| TV Show | 2015 | The Muppets | Self | 1 |
| TV Show | 2022 | RuPaul's Drag Race UK vs The World | Self - Host | 14 |
| TV Show | 2020 | Canada's Drag Race | Self - RuMail | 47 |
| TV Show | 2012 | RuPaul's Drag Race All Stars | Self - Host / Judge | 101 |
| TV Show | 2024 | RuPaul's Drag Race Global All Stars | Self - Host / Judge | 12 |
| TV Show | 2016 | The Real O'Neals | RuPaul | 1 |
| TV Show | 2011 | 2 Broke Girls | Himself | 1 |
| TV Show | 1998 | Hercules | Rock Guardian (voice) | 1 |
| TV Show | 2004 | Project Runway | Self - Guest Judge | 1 |
| TV Show | 2015 | Harvey Beaks | Jackie Slitherstein (voice) | 2 |
| TV Show | 2016 | Animals. | Dr. Labcoat | 2 |
| TV Show | 1984 | MTV Video Music Awards | Self | 1 |
| TV Show | 2014 | Hey Qween! | Self | 1 |
| TV Show | 2012 | RuPaul's Drag Race All Stars: UNTUCKED | Self - Narrator | 74 |
| TV Show | 2021 | Celebrity Wheel of Fortune | Self - Contestant | 1 |
| TV Show | 2014 | The Tonight Show Starring Jimmy Fallon | Self | 1 |
| TV Show | 2014 | Broad City | Marcel | 3 |
| TV Show | 2013 | Alyssa's Secret | Self - Guest | 1 |
| TV Show | 2014 | Late Night with Seth Meyers | Self | 2 |
| TV Show | 1996 | The Rosie O'Donnell Show | Self | 1 |
| TV Show | 2009 | Watch What Happens Live with Andy Cohen | Self - Guest | 10 |
| TV Show | 2016 | The $100,000 Pyramid | Self | 2 |
| TV Show | 2010 | RuPaul's Drag Race: Untucked | Self - Narrator | 196 |
| TV Show | 2009 | Under the Hood | Self - Narrator (voice) (uncredited) | 7 |
| TV Show | 2016 | Martha & Snoop's Potluck Dinner Party | Self | 1 |
| TV Show | 2020 | AJ and the Queen | Robert Lee / Ruby Red | 10 |
| TV Show | 2014 | Trailblazer Honors | Self - Speaker | 1 |
| TV Show | 2015 | The Late Show with Stephen Colbert | Self | 2 |
| TV Show | 2022 | Celebrity Lingo | Self - Host | 7 |
| TV Show | 1997 | The View | Self | 1 |
| TV Show | 2007 | The Graham Norton Show | Self - Guest | 1 |
| TV Show | 2016 | To Tell the Truth | Self - Panelist | 1 |
| TV Show | 2019 | RuPaul | Self - Host | 15 |
| TV Show | 2016 | Kocktails With Khloé | Self | 1 |
| TV Show | 2016 | Match Game | Self - Panelist | 1 |
| TV Show | 2019 | The X Change Rate | Self - Special Guest | 1 |
| TV Show | 2019 | RuPaul's Drag Race UK | Self - Host / Judge | 58 |
| TV Show | 2022 | Canada's Drag Race: Canada vs The World | Self - RuMail | 12 |
| TV Show | 1975 | Saturday Night Live | Self - Cameo (uncredited) | 1 |
| TV Show | 2015 | The Late Show with Stephen Colbert | Self - Guest | 1 |
| TV Show | 2005 | The Comeback | | 1 |
| TV Show | 2014 | BoJack Horseman | Queen Antonia (voice) | 1 |
| TV Show | 2007 | The Graham Norton Show | Self | 2 |
| TV Show | 2023 | Lingo | Self - Host | 28 |
| TV Show | 2015 | Grace and Frankie | Benjamin Le Day | 2 |
| TV Show | 1989 | The Simpsons | Queen Chante (voice) | 1 |
| TV Show | 2015 | Close Up with The Hollywood Reporter | Self | 1 |
| TV Show | 1975 | Saturday Night Live | Self - Host | 1 |
| TV Show | 2019 | The World's Best | Judge | 7 |
| TV Show | 2020 | Secret Celebrity RuPaul's Drag Race | Self - Host | 12 |
| TV Show | 2011 | Happy Endings | KrisJahn | 1 |
| TV Show | 2020 | Muppets Now | Self | 1 |
| TV Show | 1997 | Veronica's Closet | Brett | 1 |
| TV Show | 2016 | Skin Wars: Fresh Paint | Self - Host | 9 |
| TV Show | 2007 | Rick & Steve: The Happiest Gay Couple in All the World | Tyler (voice) | 1 |
| TV Show | 2015 | Adam Ruins Everything | | 1 |
| TV Show | 2016 | Not Today, Bianca | RuPaul | 2 |
| TV Show | 1998 | V.I.P. | Pepe Dutille | 1 |
| TV Show | 2003 | Jimmy Kimmel Live! | Self - Guest Host | 4 |
| TV Show | 2006 | The One Show | Self | 1 |
| TV Show | 2025 | Hollywood Squares | Self | 2 |
| TV Show | 2015 | RuPaul's Drag Race: RuVealed | Self | 81 |
