| Movie | 2004 | Bring It On Again | Penelope | |
| Movie | 2018 | We Brought Back MST3K | Self | |
| Movie | 2004 | June | June Marie Jacobs | |
| Movie | 2008 | Prairie Fever | Blue | |
| Movie | 2010 | Red: Werewolf Hunter | Virginia Sullivan | |
| Movie | 2012 | Rock Jocks | Alison | |
| Movie | 2006 | God's Waiting List | Trixie | |
| Movie | N/A | Backslide | Maddie | |
| Movie | 2007 | The Making of Dr. Horrible's Sing-Along Blog | Self | |
| Movie | 2017 | We Love You, Sally Carmichael! | Sarah | |
| Movie | 2014 | Lust for Love | Mary | |
| Movie | 2008 | Dr. Horrible's Sing-Along Blog | Penny | |
| Movie | 2025 | Tim Travers & the Time Travelers Paradox | Delilah | |
| Movie | 2002 | They Shoot Divas, Don't They? | Call Girl | |
| Movie | 2018 | Stuck | Robin | |
| Movie | 2005 | Mystery Woman: Vision of a Murder | Emily Claire | |
| Movie | 2019 | Chasing Molly | Kaitlin | |
| Movie | 2008 | Commentary! The Musical | | |
| Movie | 2008 | Dear Me | Pipsy | |
| Movie | 2020 | Pooka Lives! | Molly | |
| Movie | 2013 | How Videogames Changed the World | Self | |
| Movie | 2005 | Warm Springs | Eloise Hutchinson | |
| Movie | 2020 | The Candlenights 2020 Special | Self | |
| Movie | 2024 | The Ugly Chickens | Paula | |
| Movie | 2014 | 8-bit Haunted Dance Party | | |
| Movie | 2011 | Chronicles of Humanity: Descent | Amanda Wood (voice) | |
| Movie | 2022 | 1982: Greatest Geek Year Ever! | Self | |
| TV Show | 2004 | House | Apple | 1 |
| TV Show | 2001 | One on One | Sarah | 1 |
| TV Show | 2005 | Love, Inc. | Natalie | 1 |
| TV Show | 2008 | Dr. Horrible's Sing-Along Blog | Penny | 3 |
| TV Show | 2004 | Century City | Sheryl | 9 |
| TV Show | 2006 | Windfall | | 1 |
| TV Show | 2015 | We Bare Bears | Karla (voice) | 1 |
| TV Show | 2012 | Good Mythical Morning | Self | 1 |
| TV Show | 2005 | Supernatural | Charlie Bradbury | 12 |
| TV Show | 2009 | Three Rivers | | 1 |
| TV Show | 2007 | The Guild | Codex | 70 |
| TV Show | 2009 | Roommates | Alyssa | 13 |
| TV Show | 2002 | For the People | Nicole | 18 |
| TV Show | 2008 | The Legend of Neil | Fairy | 20 |
| TV Show | 2010 | Generator Rex | | 1 |
| TV Show | 1997 | Buffy the Vampire Slayer | Vi | 8 |
| TV Show | 2011 | Dragon Age: Redemption | Tallis | 6 |
| TV Show | 2010 | The Jeff Lewis 5 Minute Comedy Hour | Office Worker | 19 |
| TV Show | 2012 | The Flog | Self | 55 |
| TV Show | 2018 | Nailed It! | Self - Judge | 1 |
| TV Show | 2001 | Emeril | | 1 |
| TV Show | 2010 | My Little Pony: Friendship Is Magic | Pear Butter (voice) | 1 |
| TV Show | 2012 | MyMusic | Gorgol | 1 |
| TV Show | 2002 | Monk | Mrs. Heidi Gefsky | 1 |
| TV Show | 2006 | Eureka | Holly Marten | 18 |
| TV Show | 2017 | Mystery Science Theater 3000 | Kinga Forrester | 20 |
| TV Show | 2022 | The Legend of Vox Machina | Captain / Bandit 2 (voice) | 2 |
| TV Show | 1989 | Mystery Science Theater 3000 | Kinga Forrester | 0 |
| TV Show | 2023 | Adventure Time: Fionna & Cake | Betty (voice) | 3 |
| TV Show | 2023 | Greatest Geek Year Ever! 1982 | Self - Guest | 4 |
| TV Show | 2010 | Fish Hooks | Angela (voice) | 3 |
| TV Show | 2015 | The Magicians | Poppy | 4 |
| TV Show | 2022 | Mystery Science Theater 3000 | Kinga Forrester | 13 |
| TV Show | 2010 | The Webventures of Justin and Alden | Self | 2 |
| TV Show | 2012 | TableTop | Self - Guest | 10 |
| TV Show | 2017 | Danger & Eggs | Francesca (voice) | 1 |
| TV Show | 2022 | Monster High | Ghoulia Yelps (voice) | 10 |
| TV Show | 2017 | Marvel's Spider-Man | Mary Jane (voice) | 1 |
| TV Show | 2009 | Dollhouse | Mag | 1 |
| TV Show | 2015 | Critical Role | Lyra | 2 |
| TV Show | 2018 | The Rookie | Dr. Morgan | 2 |
| TV Show | 2000 | Strong Medicine | Jesse's Friend | 2 |
| TV Show | 2010 | Adventure Time | Betty (voice) | 2 |
| TV Show | 2015 | Kittens in a Cage | Young Josephine | 1 |
| TV Show | 2012 | The High Fructose Adventures of Annoying Orange | Peach / Ginger (voice) | 64 |
| TV Show | 2015 | Con Man | Karen | 5 |
| TV Show | 2014 | The Librarians | Charlotte | 1 |
| TV Show | 2024 | Rhett & Link's Wonderhole | Draven the Technonaut (voice) | 1 |
| TV Show | 2014 | The Wil Wheaton Project | Self | 1 |
| TV Show | 2012 | My Gimpy Life | Felicia | 3 |
| TV Show | 2013 | Outlands | 84 (voice) | 7 |
