| Movie | 1997 | White Lies | Art Hoarder | |
| Movie | 2004 | Happy Birthday Oscar Wilde | Self | |
| Movie | 2022 | Bros | Louis | |
| Movie | 1999 | Jump | Dish Macense | |
| Movie | 1983 | The Demon Murder Case | The Demon (voice) | |
| Movie | 1997 | Three Little Pigs | The Big Bad Wolf (voice) | |
| Movie | 2004 | Happy to Be Nappy and Other Stories of Me | Self (voice) | |
| Movie | 2006 | The Year Without a Santa Claus | Heat Miser | |
| Movie | 1994 | Stonewall 25: Global Voices of Pride and Protest | | |
| Movie | 1990 | The American Film Institute Presents: TV or Not TV? | Self | |
| Movie | 1993 | An Evening with Lily Tomlin and Friends | Self | |
| Movie | 2019 | Fiddler: A Miracle of Miracles | Self | |
| Movie | 2017 | Animal Crackers | Esmerelda (voice) | |
| Movie | 2016 | Hairspray Live! | Edna Turnblad | |
| Movie | 2015 | The Making of the Wiz Live! | Self | |
| Movie | 2012 | Foodfight! | Fat Cat Burglar (voice) | |
| Movie | 2007 | ShowBusiness: The Road to Broadway | Self | |
| Movie | 2007 | Words and Music by Jerry Herman | Self | |
| Movie | 2006 | Farce of the Penguins | Sheila (voice) | |
| Movie | 2006 | The Best of The Tony Awards: The Plays | Host | |
| Movie | 2005 | Broadway's Lost Treasures III: The Best of The Tony Awards | Self - Host | |
| Movie | 2004 | Mulan II | Yao (voice) | |
| Movie | 2004 | Superstar in a Housedress: The Life and Legend of Jackie Curtis | Self | |
| Movie | 2003 | Duplex | Kenneth | |
| Movie | 2003 | The Golden Girls: Lifetime Intimate Portrait Series | Self | |
| Movie | 2002 | Death to Smoochy | Merv Green | |
| Movie | 2000 | Playing Mona Lisa | Bennett | |
| Movie | 2000 | Common Ground | Don | |
| Movie | 1998 | Safe Men | Good Stuff Leo | |
| Movie | 1998 | Mulan | Yao (voice) | |
| Movie | 1996 | Sesame Street: Elmo Saves Christmas | Easter Bunny | |
| Movie | 1997 | Kull the Conqueror | Juba | |
| Movie | 1996 | Everything Relative | The Moyle | |
| Movie | 1996 | Independence Day | Marty Gilbert | |
| Movie | 1996 | The Celluloid Closet | Self | |
| Movie | 1995 | Dr. Jekyll and Ms. Hyde | Yves DuBois | |
| Movie | 1994 | Bullets Over Broadway | Sid Loomis | |
| Movie | 1993 | Mrs. Doubtfire | Frank | |
| Movie | 1993 | The Harvest | Bob Lakin | |
| Movie | 1988 | Tidy Endings | Arthur | |
| Movie | 1988 | Torch Song Trilogy | Arnold Beckoff | |
| Movie | 1988 | MaMa's Pushcart | | |
| Movie | 1986 | Apology | The derelict | |
| Movie | 1984 | Garbo Talks | Bernie Whitlock | |
| Movie | 1984 | The Times of Harvey Milk | Narrator (voice) | |
| Movie | 1999 | Double Platinum | Gary Millstein | |
| Movie | 1999 | The Sissy Duckling | Elmer (voice) | |
| Movie | 1996 | The Making of 'Independence Day' | Self | |
| Movie | 2020 | Disarm Hate | Narrator | |
| Movie | 2014 | Russian Broadway Shut Down | | |
| Movie | 2001 | Rescued from the Closet | Self | |
| Movie | 2002 | Death to Smoochy: Behind the Scenes Documentary | Self | |
| Movie | 2020 | Shoshana Bean and Friends: An Evening of Broadway | Performer | |
| Movie | 1991 | In The Shadow Of Love: A Teen AIDS Story | Andrew | |
| TV Show | 2005 | How I Met Your Mother | Lily's Smoking Voice (voice) | 1 |
| TV Show | 2016 | Full Frontal with Samantha Bee | Self | 1 |
| TV Show | 1999 | Family Guy | Buster Keaton (voice) | 1 |
| TV Show | 1993 | Intimate Portrait | Self | 1 |
| TV Show | 1998 | Hercules | Argus Panoptes (voice) | 1 |
| TV Show | 1956 | Tony Awards | Self - Winner | 1 |
| TV Show | 2014 | Late Night with Seth Meyers | Self | 1 |
| TV Show | 1995 | Happily Ever After: Fairy Tales for Every Child | Mrs. Leaperman (voice) | 1 |
| TV Show | 2009 | Watch What Happens Live with Andy Cohen | Self - Guest | 3 |
| TV Show | 2014 | Trailblazer Honors | Self - Honoree | 1 |
| TV Show | 1989 | The Simpsons | Karl (voice) | 1 |
| TV Show | 1999 | Family Guy | Tracy (voice) | 1 |
| TV Show | 1994 | Daddy's Girls | Dennis Sinclair | 13 |
| TV Show | 1984 | Miami Vice | Benedict | 1 |
| TV Show | 1975 | Saturday Night Live | Self - Cameo (uncredited) | 1 |
| TV Show | 2012 | Smash | Harvey Fierstein | 1 |
| TV Show | 2014 | BoJack Horseman | Harvey Fierstein (voice) | 1 |
| TV Show | 2017 | Big Mouth | Older Guy Town Resident (voice) | 1 |
| TV Show | 1982 | Cheers | Mark Newberger | 1 |
| TV Show | 1984 | Murder, She Wrote | Stan Hatter | 1 |
| TV Show | 1994 | Ellen | Harvey Fierstein | 1 |
| TV Show | 2009 | The Good Wife | Judge Francis Flamm | 1 |
| TV Show | 2019 | The Dark Crystal: Age of Resistance | skekAyuk (The Gourmand) (voice) | 7 |
