| Movie | 2001 | Wet Hot American Summer | Susie | |
| Movie | 2006 | Tenacious D in The Pick of Destiny | Truck Stop Waitress | |
| Movie | 2004 | Mean Girls | Mrs. George | |
| Movie | 2004 | Envy | Natalie Vanderpark | |
| Movie | 2007 | Southland Tales | Veronica Mung / Dream | |
| Movie | 2009 | Alvin and the Chipmunks: The Squeakquel | Eleanor (voice) | |
| Movie | 2008 | Baby Mama | Angie Ostrowiski | |
| Movie | 2008 | Horton Hears a Who! | Sally O'Malley (voice) | |
| Movie | 2007 | Blades of Glory | Fairchild Van Waldenberg | |
| Movie | 2004 | Wake Up, Ron Burgundy: The Lost Movie | Bank Teller | |
| Movie | 2007 | Mr. Woodcock | Maggie | |
| Movie | 2021 | Kelly Clarkson Presents: When Christmas Comes Around | Self | |
| Movie | 2007 | On Broadway | Farrah | |
| Movie | 2006 | Man of the Year | Amy Poehler | |
| Movie | 2006 | The Ex | Carol Lane | |
| Movie | 2002 | Martin & Orloff | Patty | |
| Movie | 2009 | Monsters vs Aliens | Computer (voice) | |
| Movie | 2011 | Fight for Your Right Revisited | Café Patron | |
| Movie | 2023 | Carol Burnett: 90 Years of Laughter + Love | Self - Guest | |
| Movie | 2009 | Spring Breakdown | Gayle O'Brien | |
| Movie | 2011 | Alvin and the Chipmunks: Chipwrecked | Eleanor (voice) | |
| Movie | 2012 | Betty White's 90th Birthday: A Tribute to America's Golden Girl | Self | |
| Movie | 2015 | Inside Out | Joy (voice) | |
| Movie | 2014 | The Unbelievably Sweet Alpacas | Sunshine | |
| Movie | 2013 | A.C.O.D. | Sondra | |
| Movie | 2014 | They Came Together | Molly | |
| Movie | 2007 | Asssscat | | |
| Movie | 2013 | Are You Here | Terri Baker | |
| Movie | 2013 | Free Birds | Jenny (voice) | |
| Movie | 1998 | Tomorrow Night | Woman Getting Squirted | |
| Movie | 2015 | Sisters | Maura Ellis | |
| Movie | 2007 | Wild Girls Gone | Doreen Moran | |
| Movie | 2013 | Anchorman 2: The Legend Continues | Wendy Van Peele (uncredited) | |
| Movie | 2023 | First Time Female Director | Meg | |
| Movie | 2014 | Don Rickles: One Night Only | Self | |
| Movie | 2010 | Freak Dance | Lilian | |
| Movie | 2022 | Norman Lear: 100 Years of Music and Laughter | Self | |
| Movie | 2024 | Inside Out 2 | Joy (voice) | |
| Movie | 2009 | Saturday Night Live: The Best of Amy Poehler | Various (archive footage) | |
| Movie | 2001 | North Hollywood | Wendy | |
| Movie | 2024 | I'm "George Lucas": A Connor Ratliff Story | Self (archive footage) | |
| Movie | 2015 | Live from New York! | Self | |
| Movie | 1999 | Deuce Bigalow: Male Gigolo | Ruth | |
| Movie | 2017 | The House | Kate Johansen | |
| Movie | 2015 | Hurricane of Fun: The Making of Wet Hot | Self | |
| Movie | 1996 | Escape From a Wonderful Life | Mary Hatch (voice) | |
| Movie | 2015 | A Very Murray Christmas | Liz | |
| Movie | 2015 | Mind Candy | Joy (voice) | |
| Movie | 1998 | Ugly Revenge | Pretty Lady | |
| Movie | 2015 | Thank You, Del: The Story of the Del Close Marathon | Self | |
| Movie | 2011 | Hoodwinked Too! Hood VS. Evil | Gretel (voice) | |
| Movie | 2007 | The Making of 'The Pick of Destiny' | Self | |
| Movie | 2016 | Norman Lear: Just Another Version of You | Self | |
| Movie | 2013 | Never Before, Never Again: Behind the Scenes of Asssscat | Self | |
| Movie | 2007 | Shrek the Third | Snow White (voice) | |
| Movie | 2008 | Hamlet 2 | Cricket Feldstein | |
| Movie | 2018 | Love, Gilda | Self | |
| Movie | 2019 | Wine Country | Abby | |
| Movie | 2016 | Comedy Central Roast of Rob Lowe | The Devil | |
| Movie | 2010 | The Women of SNL | Various (archive footage) | |
| Movie | 2020 | A Parks and Recreation Special | Leslie Knope | |
| Movie | 2020 | Stronger Together, Tous Ensemble | Self | |
| Movie | 2007 | Shortcut to Happiness | Molly Gilchrest | |
| Movie | 2005 | SpongeBob SquarePants: Where's Gary? | Grandma | |
| Movie | 2020 | The Paley Center Salutes Parks and Recreation | Leslie Knope | |
| Movie | 2015 | Riley's First Date? | Joy (voice) | |
| Movie | 2021 | Moxie | Lisa Carter | |
| Movie | 2006 | Tenacious D: For the Ladies | | |
| Movie | 2020 | One World: Together at Home | Self | |
| Movie | 2005 | Saving Manhattan | Kristen | |
| Movie | 2000 | Zoe Loses It | Pink | |
| Movie | 2003 | Sick in the Head | | |
| Movie | 2009 | The Mystery of Claywoman | Celeste Dupree | |
| Movie | 2007 | Girl Missing | Vikki | |
| Movie | 2017 | Household Name | | |
| Movie | 2025 | SNL50: The Homecoming Concert | Self | |
| Movie | N/A | Inside Out 3 | Joy (voice) | |
| TV Show | 1989 | The Simpsons | Jenda (voice) | 2 |
| TV Show | 2003 | The Ellen DeGeneres Show | Self | 2 |
| TV Show | 2005 | Wonder Showzen | | 1 |
| TV Show | 1975 | Saturday Night Live | Self - Various Characters | 138 |
| TV Show | 1988 | LIVE with Kelly and Mark | | 1 |
| TV Show | 1996 | Spin City | Susan | 1 |
| TV Show | 1944 | Golden Globe Awards | Self - Host | 4 |
| TV Show | 1992 | The Tonight Show with Jay Leno | Self | 1 |
| TV Show | 2001 | Undeclared | Hillary | 2 |
| TV Show | 1983 | Friday Night Videos | | 1 |
| TV Show | 2012 | The Greatest Event in Television History | Jennifer Hart | 1 |
| TV Show | 1998 | Upright Citizens Brigade | Colby | 30 |
| TV Show | 2009 | The Jay Leno Show | Self | 1 |
| TV Show | 1993 | Late Night with Conan O'Brien | Self - Guest | 8 |
| TV Show | 2003 | Arrested Development | Gob's Wife | 5 |
| TV Show | 2008 | Saturday Night Live Weekend Update Thursday | | 9 |
| TV Show | 2007 | The Graham Norton Show | Self | 2 |
| TV Show | 2009 | Parks and Recreation | Leslie Knope | 122 |
| TV Show | 2004 | O'Grady | | 1 |
| TV Show | 2008 | The Mighty B! | Bessie Higgenbottom | 75 |
| TV Show | 2023 | The Gentle Art of Swedish Death Cleaning | Narrator (voice) | 8 |
| TV Show | 2005 | Weekends at the D.L. | | 1 |
| TV Show | 1975 | Saturday Night Live | Self - Cameo (uncredited) | 11 |
| TV Show | 2015 | Wet Hot American Summer: First Day of Camp | Susie | 8 |
| TV Show | 2014 | Late Night with Seth Meyers | Self | 12 |
| TV Show | 1999 | SpongeBob SquarePants | Grandma (voice) | 1 |
| TV Show | 2014 | Broad City | Cheryl | 1 |
| TV Show | 1975 | Saturday Night Live | Self - Host | 2 |
| TV Show | 2011 | Billy on the Street | Self | 2 |
| TV Show | 2016 | The UCB Show | Self - Host | 8 |
| TV Show | 2016 | America Divided | Self | 8 |
| TV Show | 1997 | The View | Self - Guest | 1 |
| TV Show | 2006 | 30 Rock | Young Liz | 1 |
| TV Show | 2014 | The Tonight Show Starring Jimmy Fallon | Self | 1 |
| TV Show | 2017 | Wet Hot American Summer: Ten Years Later | Susie | 8 |
| TV Show | 2018 | Making It | Self - Host | 22 |
| TV Show | 2020 | Duncanville | Leslie Knope (voice) | 1 |
| TV Show | 2019 | The Kelly Clarkson Show | | 1 |
| TV Show | 2014 | Welcome to Sweden | Amy Poehler | 8 |
| TV Show | 1997 | The View | Self | 1 |
| TV Show | 2019 | Desus & Mero | Self - Illustrious Guest | 1 |
| TV Show | 2021 | Baking It | Self - Host | 6 |
| TV Show | 2020 | Duncanville | Duncan / Annie (voice) | 33 |
| TV Show | 1975 | Saturday Night Live | Self | 1 |
| TV Show | 2003 | Comedy Central Roasts | Self | 1 |
| TV Show | 2003 | Jimmy Kimmel Live! | Self | 4 |
| TV Show | 2005 | Wonder Showzen | Miss Mary | 1 |
| TV Show | 1953 | The Oscars | Self | 1 |
| TV Show | 2012 | Comedy Bang! Bang! | Self | 1 |
| TV Show | 2013 | Hollywood Game Night | Self | 1 |
| TV Show | 2019 | The Kelly Clarkson Show | Self | 1 |
| TV Show | 2009 | Late Night with Jimmy Fallon | Self | 1 |
| TV Show | 2010 | Conan | Self | 1 |
| TV Show | 2018 | Lie Detector | Self | 1 |
| TV Show | 2013 | The Last Leg | Self | 1 |
| TV Show | 2024 | Dream Productions | Joy (voice) | 4 |
| TV Show | 2025 | SNL50: Beyond Saturday Night | Self | 4 |
| TV Show | 1953 | The Oscars | Self - Presenter | 1 |
| TV Show | 2025 | Good Hang with Amy Poehler | Self | 22 |
| TV Show | N/A | Dig | | 1 |
| TV Show | 2009 | The Tonight Show with Conan O'Brien | Self | 1 |
| TV Show | 2015 | Difficult People | Flute | 1 |
