| Movie | 2023 | Wonka | Prodnose | |
| Movie | 2010 | Alice in Wonderland | Tweedledee / Tweedledum | |
| Movie | 2004 | AD/BC: A Rock Opera | God | |
| Movie | 2005 | Cold & Dark | Dr. Elgin | |
| Movie | 2010 | The Infidel | Rabbi | |
| Movie | 2006 | The Wind in the Willows | Toad | |
| Movie | 2011 | Gnomeo & Juliet | Benny (voice) | |
| Movie | 2006 | Little Britain Live | Various | |
| Movie | 2010 | The One Ronnie | | |
| Movie | 2010 | Les Mis at 25: Matt Lucas Dreams the Dream | Presenter | |
| Movie | 2024 | Gladiator II | Master of Ceremonies | |
| Movie | 2011 | Derren Brown: Behind the Mischief | Himself | |
| Movie | 2006 | When Comedy Changed Forever | Self | |
| Movie | 2024 | We Work for the Dead | Perry (voice) | |
| Movie | 2011 | Bridesmaids | Gil | |
| Movie | 2012 | Small Apartments | Franklin Franklin | |
| Movie | 2010 | Les Misérables: 25th Anniversary in Concert | Thenardier | |
| Movie | 2013 | The Look of Love | Divine | |
| Movie | 2004 | The Real Little Britain | Narrator | |
| Movie | 2013 | In Secret | Olivier | |
| Movie | 2003 | Moo(n) | Bee | |
| Movie | 1999 | Plunkett & MacLeane | Sir Oswald | |
| Movie | 2022 | Ian Cognito: A Life and A Death On Stage | Self | |
| Movie | 2014 | Paddington | Joe | |
| Movie | 2005 | Little Britain Comic Relief | Andy | |
| Movie | 2021 | Friday Night Dinner: 10 Years and a Lovely Bit of Squirrel | Narrator (voice) | |
| Movie | 2017 | How to Talk to Girls at Parties | PT Wain | |
| Movie | 2016 | Alice Through the Looking Glass | Tweedledee / Tweedledum | |
| Movie | 2015 | Doctor Who: The Husbands of River Song | Nardole | |
| Movie | 2015 | Peter Kay: 20 Years of Funny | Self | |
| Movie | 1995 | B-Roads | Self | |
| Movie | 1998 | Jilting Joe | Air Steward | |
| Movie | 2018 | A Futile and Stupid Gesture | Tony Hendra | |
| Movie | N/A | Flower of the Dawn | Zachiel (voice) | |
| Movie | 2016 | A Midsummer Night's Dream | Bottom | |
| Movie | 2009 | Now That’s What I Call Comedy | Andy Pipkin | |
| Movie | 2016 | The Labyrinth | Sam | |
| Movie | 2004 | Shaun of the Dead | Cousin Tom | |
| Movie | 2002 | Surrealissimo: The Trial of Salvador Dali | Luis Bunuel | |
| Movie | 2017 | Cabiria, Charity, Chastity | Panhandler | |
| Movie | 2012 | Britain's Gay Footballers | Himself | |
| Movie | 2017 | Doctor Who: Twice Upon a Time | Nardole | |
| Movie | 2019 | Polar | Blut | |
| Movie | 2016 | Derren Brown: Pushed to the Edge | Himself | |
| Movie | 2009 | Astro Boy | Sparx (voice) | |
| Movie | 2018 | Sherlock Gnomes | Benny (voice) | |
| Movie | 2019 | Missing Link | Mr. Collick (voice) | |
| Movie | 2017 | Proclaimers: This Is the Story | Self | |
| Movie | 2019 | The Queen's Corgi | Charlie (voice) | |
| Movie | 2018 | Aliens Love Underpants and...Panta Claus | | |
| Movie | 2013 | The Harry Hill Movie | Otto | |
| Movie | 1998 | You Are Here | Pat Magnet | |
| Movie | 2003 | Welcome to Glaringly | Rod | |
| Movie | 2019 | Les Misérables: The Staged Concert | Thénardier | |
| Movie | 2016 | Doctor Who: The Return of Doctor Mysterio | Nardole | |
| Movie | N/A | Molesworth | Nigel Molesworth (voice) | |
| Movie | N/A | Come Fly on the Wall | Self | |
| Movie | 2014 | The Nation's Favourite Queen Song | Narrator | |
| Movie | 2012 | Freddie Mercury: The Great Pretender | | |
| Movie | 2021 | 50 Years of Mr Men with Matt Lucas | Self - Presenter | |
| Movie | 1997 | Dennis Pennis R.I.P. | 'The Quill' | |
| Movie | 2007 | Little Britain Down Under | Self | |
| Movie | 2005 | Alan Partridge Presents: The Cream of British Comedy | Daffydd Thomas | |
| Movie | 2025 | An Evening With Elton John and Brandi Carlile | Self - Audience Member | |
| Movie | 2007 | The Bluetones: Blue Movies | Record Company Boss | |
| TV Show | 2005 | Popetown | Cardinal One (voice) | 10 |
| TV Show | 1988 | This Morning | Self | 1 |
| TV Show | 1993 | Shooting Stars | George Dawes | 57 |
| TV Show | 2004 | Catterick | Roy Oates | 6 |
| TV Show | 2005 | Monkey Trousers | | 7 |
| TV Show | 2006 | The One Show | Self | 3 |
| TV Show | 2001 | Friday Night with Jonathan Ross | Self | 2 |
| TV Show | 2005 | King Arthur's Disasters | Merlin | 23 |
| TV Show | 1978 | The South Bank Show | Self | 1 |
| TV Show | 2020 | The Masked Singer | Self - Guest Panelist | 1 |
| TV Show | 2000 | Randall & Hopkirk (Deceased) | | 1 |
| TV Show | 2002 | Kath & Kim | Karen | 2 |
| TV Show | 2018 | The Great Celebrity Bake Off for Stand Up To Cancer | Self - Host | 15 |
| TV Show | 2007 | The Dame Edna Treatment | | 1 |
| TV Show | 1999 | Bang, Bang, It's Reeves and Mortimer | | 6 |
| TV Show | 1999 | Rock Profile | Gary Barlow | 29 |
| TV Show | 1993 | The Smell of Reeves and Mortimer | Mayor Hobson | 12 |
| TV Show | 2005 | Casanova | Villars | 3 |
| TV Show | 2016 | DC's Legends of Tomorrow | Aleister Crowley (voice) | 2 |
| TV Show | 2007 | Katie & Peter: Unleashed | | 1 |
| TV Show | 2008 | Little Britain USA | | 6 |
| TV Show | 2009 | Totally Saturday | | 1 |
| TV Show | 2004 | Derren Brown: Trick of the Mind | | 1 |
| TV Show | 1997 | Sunnyside Farm | Mr. Mills | 6 |
| TV Show | 1999 | Sir Bernard's Stately Homes | Sir Bernard Chumley | 6 |
| TV Show | 2009 | Krod Mandoon and the Flaming Sword of Fire | Chancellor Dongalor | 6 |
| TV Show | 2012 | The Matt Lucas Awards | | 12 |
| TV Show | 2010 | Comedy Rocks with Jason Manford | | 1 |
| TV Show | 2015 | Man Seeking Woman | Igor | 1 |
| TV Show | 2010 | Come Fly with Me | Fearghal O'Farrell | 6 |
| TV Show | 2013 | Super Fun Night | Derrick | 2 |
| TV Show | 2009 | Community | Toby | 1 |
| TV Show | 2019 | RuPaul's Drag Race UK | Self - Guest Judge | 1 |
| TV Show | 2015 | Pompidou | Pompidou | 6 |
| TV Show | 1987 | French & Saunders | | 1 |
| TV Show | 2005 | Doctor Who | Nardole | 12 |
| TV Show | 1996 | Mash and Peas | | 9 |
| TV Show | 2007 | Gavin & Stacey | Jammy | 1 |
| TV Show | 2014 | Late Night with Seth Meyers | Self | 1 |
| TV Show | 2006 | The One Show | Self - Guest | 1 |
| TV Show | 2007 | The Graham Norton Show | Self | 7 |
| TV Show | 2014 | Alan Davies: As Yet Untitled | Self | 1 |
| TV Show | 2004 | Who Do You Think You Are? | | 1 |
| TV Show | 2015 | Harvey Beaks | Lady Paddles (voice) | 1 |
| TV Show | 2003 | QI | Self | 6 |
| TV Show | 2011 | Portlandia | Stu | 2 |
| TV Show | 2013 | Gogglebox | Self | 1 |
| TV Show | 2015 | Travel Man: 48 Hours in... | Self - Guest | 1 |
| TV Show | 2013 | Julia Zemiro's Home Delivery | Self | 1 |
| TV Show | 1992 | The Tonight Show with Jay Leno | | 1 |
| TV Show | 2017 | The Russell Howard Hour | Self | 1 |
| TV Show | 2017 | The Great British Bake Off: An Extra Slice | Self | 3 |
| TV Show | 2012 | Honest Trailers | Prodnose (archive footage) | 1 |
| TV Show | 2002 | Ant & Dec's Saturday Night Takeaway | Self - Guest Announcer | 1 |
| TV Show | 2022 | Fantasy Football League | Self - Host | 24 |
| TV Show | 2006 | The Russell Brand Show | Self | 1 |
| TV Show | 2003 | Little Britain | Various Roles | 20 |
| TV Show | 2018 | The Big Narstie Show | Self | 1 |
| TV Show | 2017 | Bill Nye Saves the World | Salted Caramel (voice) | 1 |
| TV Show | 2015 | Fresh Off the Boat | Mr. Fisher | 1 |
| TV Show | 2014 | Monty Python's Best Bits (Mostly) | Self | 1 |
| TV Show | 2017 | The Great British Bake Off | Self - Presenter | 30 |
| TV Show | 2000 | Lum the Invader Girl | Ataru Moroboshi (voice) | 2 |
| TV Show | 2013 | The Last Leg | Self | 1 |
| TV Show | 1962 | The Late Late Show | Self | 1 |
| TV Show | 2002 | Look Around You | Dr. Phillip Lavender | 2 |
| TV Show | 1967 | Omnibus | Self | 1 |
