| Movie | 1982 | Star Trek II: The Wrath of Khan | Cmdr. Uhura | |
| Movie | 1979 | Star Trek: The Motion Picture | Lt. Cmdr. Uhura | |
| Movie | 1984 | Star Trek III: The Search for Spock | Cmdr. Uhura | |
| Movie | 1986 | Star Trek IV: The Voyage Home | Uhura | |
| Movie | 1989 | Star Trek V: The Final Frontier | Cmdr. Uhura | |
| Movie | 1991 | Star Trek VI: The Undiscovered Country | Cmdr. Uhura | |
| Movie | 2008 | The Torturer | | |
| Movie | 1967 | Tarzan's Jungle Rebellion | Ruana | |
| Movie | 1974 | Truck Turner | Dorinda | |
| Movie | 2002 | Snow Dogs | Amelia | |
| Movie | 2020 | Star Trek: First Frontier | Nyota Uhura | |
| Movie | 2003 | Roddenberry on Patrol | Grace | |
| Movie | 2010 | Scooby-Doo! Curse of the Lake Monster | Senator | |
| Movie | 1986 | The Supernaturals | Sgt. Leona Hawkins | |
| Movie | 2012 | Get a Life! | Herself | |
| Movie | 1995 | The Adventures of Captain Zoom in Outer Space | Sagan | |
| Movie | 2008 | Lady Magdalene's | Lady Magdalene / Maggie, Singer | |
| Movie | 1966 | Mister Buddwing | Dice Player | |
| Movie | 2014 | To Be Takei | Self | |
| Movie | 2018 | United We Fan | Self | |
| Movie | 1996 | Star Trek Story | Self | |
| Movie | 1996 | The Last Angel of History | Self | |
| Movie | 1983 | Antony and Cleopatra | Charmain | |
| Movie | 2005 | Are We There Yet? | Miss Mable | |
| Movie | 1986 | Super Duper Bloopers | Self | |
| Movie | 2009 | Bring Back... Star Trek | Self | |
| Movie | 2020 | Unbelievable!!!!! | Sensei | |
| Movie | 2006 | Comedy Central Roast of William Shatner | Self - Roaster | |
| Movie | 2015 | Roddenberry's Trek | herself | |
| Movie | 1995 | William Shatner's Star Trek Memories | Self | |
| Movie | 2007 | Star Trek: Of Gods and Men | Captain Nyota Uhura | |
| Movie | 1970 | Tarzan's Deadly Silence | Ruana | |
| Movie | 2016 | For the Love of Spock | Self | |
| Movie | 2016 | 50 Years of Star Trek | Self | |
| Movie | 2005 | Surge of Power: The Stuff of Heroes | Oman | |
| Movie | 2016 | Building Star Trek | Herself | |
| Movie | 1994 | William Shatner: Captain's Log | Self | |
| Movie | 2016 | The Order | Athena | |
| Movie | 1991 | Star Trek: 25th Anniversary Special | Self | |
| Movie | 2017 | Renegades: The Requiem: Part 1 | Admiral Grace Jemison | |
| Movie | 1994 | Star Trek: A Captain's Log | Herself | |
| Movie | 2008 | Tru Loved | Grandmother | |
| Movie | 2017 | Sharknado 5: Global Swarming | Secretary General of NATO | |
| Movie | 2018 | The White Orchid | Teresa | |
| Movie | 2018 | American Nightmares | Mystic Woman | |
| Movie | 2004 | Kiss 'N' Tell: Romance in the 23rd Century | | |
| Movie | 2004 | Birth of a Timeless Legacy | | |
| Movie | 2007 | Star Trek: Beyond the Final Frontier | Self | |
| Movie | 2006 | 40 Years of Star Trek | Self | |
| Movie | 1997 | Trekkies | Self | |
| Movie | 1967 | Doctor, You've Got to Be Kidding! | Jenny Ribbock | |
| Movie | 1988 | The Star Trek Saga: From One Generation to the Next | Self | |
| Movie | 2016 | Star Trek: Inside the Roddenberry Vault | Self | |
| Movie | 2019 | Surge of Dawn | Omen | |
| Movie | 1993 | Commander Toad in Space | SS Stella (voice) | |
| Movie | 1959 | Porgy and Bess | Dancer | |
| Movie | 2021 | Woman in Motion | Self | |
| Movie | 1998 | Small Steps, Big Strides: The Black Experience in Hollywood | Self | |
| Movie | 1966 | Made in Paris | Eleanor | |
| Movie | 1976 | Woman in the Rain | Singer | |
| Movie | 2021 | Star Trek Renegades OMINARA | Ominara | |
| Movie | 1992 | Black Sci-Fi | Self | |
| Movie | 1966 | Star Trek: The Menagerie | Uhura | |
| Movie | 2015 | Star Trek: Renegades | Admiral Grace Jemison | |
| TV Show | 1989 | The Simpsons | Nichelle Nichols (voice) | 1 |
| TV Show | 1994 | Spider-Man | Miriam (voice) | 2 |
| TV Show | 1966 | Star Trek | Uhura | 79 |
| TV Show | 1999 | Futurama | Nichelle Nichols' Head (voice) | 2 |
| TV Show | 2006 | Heroes | Nana Dawson | 5 |
| TV Show | 1973 | Star Trek | Uhura (voice) | 22 |
| TV Show | 2012 | Comic Book Men | Self | 1 |
| TV Show | 1994 | Gargoyles | Diane Maza (voice) | 3 |
| TV Show | 1992 | Batman: The Animated Series | Thoth Khepera (voice) | 1 |
| TV Show | 2021 | The Center Seat: 55 Years of Star Trek | Self | 5 |
| TV Show | 2020 | Heaven's Gate: The Cult of Cults | Self - Sister of Member (archive footage) | 1 |
| TV Show | 1995 | Die Harald Schmidt Show | Self - Guest | 1 |
| TV Show | 2021 | Star Trek: Prodigy | Uhura (voice) | 1 |
| TV Show | 2017 | Downward Dog | Deejay Devine | 1 |
| TV Show | 2002 | After They Were Famous | Self | 1 |
| TV Show | 2000 | Buzz Lightyear of Star Command | Chief (voice) | 1 |
| TV Show | 1963 | The Lieutenant | Norma Bartlett | 1 |
| TV Show | 1984 | Goldene Kamera Verleihung | Self | 1 |
| TV Show | 2018 | One Strange Rock | Self | 1 |
| TV Show | 2003 | Comedy Central Roasts | Self | 1 |
| TV Show | 2014 | The Real History of Science Fiction | Self | 1 |
| TV Show | 2020 | Challenger: The Final Flight | Self (archive footage) | 1 |
