| Movie | 1985 | The Color Purple | Sofia | |
| Movie | 2009 | The Princess and the Frog | Eudora (voice) | |
| Movie | 2007 | Bee Movie | Judge Bumbleton (voice) | |
| Movie | 2006 | Charlotte's Web | Gussy the Goose (voice) | |
| Movie | 2021 | Adele One Night Only | Self | |
| Movie | 1997 | About Us: The Dignity of Children | Host | |
| Movie | 2001 | Quincy Jones: In the Pocket | Self | |
| Movie | 2023 | The Light We Carry: Michelle Obama and Oprah Winfrey | Self | |
| Movie | 2023 | Carol Burnett: 90 Years of Laughter + Love | Self - Guest | |
| Movie | 1998 | Beloved | Sethe | |
| Movie | 2013 | Martin Luther King and the March on Washington | Self | |
| Movie | 2022 | Soul of a Nation Presents: Screen Queens Rising | Self | |
| Movie | 2009 | My White Baby | Self (voice) (archive footage) (uncredited) | |
| Movie | 1986 | Native Son | Mrs. Thomas | |
| Movie | 1989 | The Women of Brewster Place | Mattie Michael | |
| Movie | 2013 | The Butler | Gloria Gaines | |
| Movie | 2022 | Oprah + Viola: A Netflix Special Event | Self | |
| Movie | 2021 | Parkinson at 50 | Self (archive footage) | |
| Movie | 2009 | It's Complicated | Self (uncredited) | |
| Movie | 1997 | Before Women Had Wings | Zora Williams | |
| Movie | 1987 | Throw Momma from the Train | Herself | |
| Movie | 2018 | Crow: The Legend | The One Who Creates Everything by Thinking (voice) | |
| Movie | 2023 | Satan Wants You | Self (archive footage) | |
| Movie | 2014 | Selma | Annie Lee Cooper | |
| Movie | 2022 | Sidney | Self | |
| Movie | 2015 | The 10 Faces of Michael Jackson | Self (archive footage) | |
| Movie | 2021 | Oprah Winfrey: Fight for Better Life | Herself | |
| Movie | 2023 | Oprah & The Color Purple Journey | Self | |
| Movie | 2023 | Unstuffed: A Build-A-Bear Story | Self | |
| Movie | 1988 | Pee-wee's Playhouse Christmas Special | Self | |
| Movie | 2013 | Making a Scene | Woman | |
| Movie | 1999 | Tina Turner : Celebrate! | Self | |
| Movie | 1992 | Lincoln | Elizabeth Keckley (voice) | |
| Movie | 2005 | Emmanuel's Gift | Narrator (voice) | |
| Movie | 2011 | Hey, Boo: Harper Lee & To Kill a Mockingbird | Self | |
| Movie | 2016 | An Oprah Winfrey Special: First Lady Michelle Obama Says Farewell To The White House | Self | |
| Movie | 2022 | Whitney Houston: I Wanna Dance with Somebody | Self (archive footage) | |
| Movie | 2022 | Homeward Bound: A Grammy Salute to the Songs of Paul Simon | Self | |
| Movie | 2024 | An Oprah Special: Shame, Blame and the Weight Loss Revolution | Self | |
| Movie | 2016 | Black America since MLK: And still I rise | | |
| Movie | 2024 | The Six Triple Eight | Mary McLeod Bethune | |
| Movie | 2024 | La piqûre de la minceur | Self (archive footage) (uncredited) | |
| Movie | 2013 | Propaganda | Self (archive footage) | |
| Movie | 2011 | There's Something Wrong with Aunt Diane | Self (archive footage) | |
| Movie | 2024 | South Park: The End of Obesity | Self (archive footage) (uncredited) | |
| Movie | 1999 | Our Friend, Martin | Coretta Scott King (voice) | |
| Movie | 2006 | A Special Presentation Oprah and Elie Wiesel at Auschwitz Death Camp | Self | |
| Movie | 2018 | A Wrinkle in Time | Mrs. Which | |
| Movie | 2022 | Oprah and Quinta Brunson Abbott Elementary | Herself | |
| Movie | 2014 | New World Order: Communism by the Backdoor | Self (archive footage) | |
| Movie | 2017 | The Immortal Life of Henrietta Lacks | Deborah Lacks | |
| Movie | 2012 | Jesus Henry Christ | Oprah | |
| Movie | 2016 | Before the Flood | Self (archive footage) | |
| Movie | 2024 | Diane von Furstenberg: Woman in Charge | Self | |
| Movie | 2017 | The Star | Deborah (voice) | |
| Movie | 2016 | Maya Angelou: And Still I Rise | Self | |
| Movie | 2017 | 13th: A Conversation with Oprah Winfrey & Ava DuVernay | Herself | |
| Movie | 2017 | Whitney: Can I Be Me | Self (archive footage) (uncredited) | |
| Movie | 2017 | Hand In Hand: A Benefit For Hurricane Relief | Self | |
| Movie | 2017 | Spielberg | Self | |
| Movie | 2003 | Unchained Memories: Readings from the Slave Narratives | | |
| Movie | 2007 | Ocean's Thirteen | Oprah Winfrey | |
| Movie | 2016 | Casablancas: The Man Who Loved Women | Self | |
| Movie | 2013 | Are All Men Pedophiles? | Self (archive footage) | |
| Movie | 2016 | National Museum of African American History and Culture Grand Opening Ceremony | Self | |
| Movie | 2018 | Quincy | Self | |
| Movie | 2018 | Oprah Winfrey Presents: Becoming Michelle Obama | Self - Host | |
| Movie | 2019 | Oprah Winfrey Presents: After Neverland | Self | |
| Movie | 2016 | Enlighten Us: The Rise and Fall of James Arthur Ray | Self (archive footage) | |
| Movie | 1993 | There Are No Children Here | LaJoe Rivers | |
| Movie | 2019 | Oprah Winfrey Presents: When They See Us Now | Self | |
| Movie | 2019 | Toni Morrison: The Pieces I Am | Self | |
| Movie | 2013 | The Armstrong Lie | Self (archive footage) | |
| Movie | 2013 | The Making of 'Making a Scene' | Self | |
| Movie | 2018 | Q85: A Musical Celebration for Quincy Jones | Self - Host | |
| Movie | 2020 | One World: Together at Home | Self | |
| Movie | 2020 | Uncle Tom | Self (archive footage) | |
| Movie | 2020 | Between the World and Me | Self | |
| Movie | 2020 | You Cannot Kill David Arquette | Self (archive footage) (uncredited) | |
| Movie | 2020 | Chadwick Boseman: A Tribute for a King | Self | |
| Movie | 2020 | Becoming | Self | |
| Movie | 2007 | Everything's Cool | Self (archive footage) | |
| Movie | 2017 | Get Me Roger Stone | Self (archive footage) | |
| Movie | 2019 | Mike Wallace Is Here | Self (archive footage) | |
| Movie | 2020 | The Choice 2020: Trump vs. Biden | Self (archive footage) | |
| Movie | 2021 | TINA | Self | |
| Movie | 2021 | Oprah with Meghan and Harry: A CBS Primetime Special | Self | |
| Movie | 2021 | The United States vs. Billie Holiday Special: Lee Daniels and Cast Interviewed by Oprah Winfrey | Self | |
| Movie | 2014 | Life Itself | Self - TV Host (archive footage) | |
| Movie | 2020 | The Accidental President | Self (archive footage) | |
| Movie | 2021 | The Perfect Victim | Self (archive footage) | |
| Movie | 2020 | John Lewis: Celebrating a Hero | | |
| Movie | 2002 | The Cosby Show: A Look Back | Self | |
| Movie | 2024 | AI and the Future of Us: An Oprah Winfrey Special | Self - Host | |
| Movie | 2024 | Robert Downey Jr.: High Altitude | Self | |
| Movie | 1993 | Michael Jackson Talks... to Oprah | Self | |
| Movie | 2024 | An Oprah Special: The Presleys – Elvis, Lisa Marie and Riley | Self - Host | |
| Movie | 2024 | Piece by Piece | Oprah Winfrey (voice - archival footage) | |
| Movie | 2024 | The Choice 2024: Harris vs. Trump | Self (archive footage) | |
| Movie | 2024 | Doc of Chucky | Self (archive footage) (uncredited) | |
| Movie | 2024 | OWN Spotlight: Oprah & Angela Bassett | Self | |
| Movie | 2025 | This Is the Tom Green Documentary | Self (archive footage) | |
| Movie | 2025 | Predators | Self (archive footage) | |
| Movie | N/A | Terms of Endearment | Aurora | |
| Movie | 2025 | An Oprah Winfrey Special: The Menopause Revolution | Self - Host | |
| Movie | 2025 | Barbara Walters: Tell Me Everything | Self | |
| Movie | 1992 | Surviving A Break-Up | Self | |
| Movie | 1993 | Shades Of A Single Protein | Self | |
| Movie | 1993 | Learning Not To Hurt | Self | |
| Movie | 1994 | I Hate The Way I Look | Self | |
| TV Show | 1994 | Ellen | Therapist | 2 |
| TV Show | 1990 | Gabriel's Fire | | 1 |
| TV Show | 1991 | Home Improvement | Oprah Winfrey | 1 |
| TV Show | 1997 | The View | Self | 1 |
| TV Show | 1990 | The Fresh Prince of Bel-Air | Herself | 1 |
| TV Show | 2006 | Rachael Ray | | 1 |
| TV Show | 2019 | Tamron Hall | Self - Guest | 1 |
| TV Show | 1996 | E! True Hollywood Story | | 1 |
| TV Show | 1992 | The Tonight Show with Jay Leno | | 2 |
| TV Show | 2006 | Web Junk 20 | | 1 |
| TV Show | 2003 | Anderson Cooper 360° | Self | 2 |
| TV Show | 1968 | 60 Minutes | Self | 1 |
| TV Show | 2006 | 30 Rock | Oprah Winfrey | 1 |
| TV Show | 2021 | Super Soul | Self | 7 |
| TV Show | 2008 | Oprah's Big Give | | 8 |
| TV Show | 2014 | Lindsay | Self | 8 |
| TV Show | 1982 | Late Night with David Letterman | Self | 1 |
| TV Show | 1995 | MADtv | Self | 1 |
| TV Show | 2015 | The Late Show with Stephen Colbert | Self | 1 |
| TV Show | 1998 | The Hughleys | | 1 |
| TV Show | 2004 | Drake & Josh | Oprah Winfrey | 1 |
| TV Show | 2020 | Dear... | Self | 1 |
| TV Show | 2003 | Celebrities Uncensored | Self | 1 |
| TV Show | 2021 | The Me You Can't See | Self | 6 |
| TV Show | 1990 | Brewster Place | Mattie Michael | 10 |
| TV Show | 1974 | The Daytime Emmy Awards | | 2 |
| TV Show | 2000 | Life & Cooking | Self | 1 |
| TV Show | 1953 | The Oscars | Self | 3 |
| TV Show | 1949 | The Emmy Awards | Self | 1 |
| TV Show | 2012 | Oprah's Next Chapter | Self - Host | 1 |
| TV Show | 1975 | Saturday Night Live | Self - Host | 1 |
| TV Show | 2023 | Pretty Baby: Brooke Shields | Self (archive footage) | 1 |
| TV Show | 1978 | People Are Talking | Herself - Host | 1 |
| TV Show | 2023 | 100 Years of Warner Bros. | Self | 4 |
| TV Show | 1986 | American Masters | Self | 1 |
| TV Show | 1962 | The Tonight Show Starring Johnny Carson | Self | 1 |
| TV Show | 1982 | Wogan | Self | 1 |
| TV Show | 2013 | Makers: Women Who Make America | Self | 3 |
| TV Show | 2022 | Sherri | Self - Guest | 1 |
| TV Show | 2022 | The Jennifer Hudson Show | Self | 1 |
| TV Show | 2016 | Greenleaf | Mavis McCready | 9 |
| TV Show | 1944 | Golden Globe Awards | Self - Presenter | 1 |
| TV Show | 2003 | The Ellen DeGeneres Show | Self | 3 |
| TV Show | 2012 | Oprah: Where Are They Now? | Herself - Host | 12 |
| TV Show | 1959 | The Grammys | Self | 1 |
| TV Show | 1994 | All-American Girl | Self | 1 |
| TV Show | 2003 | Jimmy Kimmel Live! | Self | 1 |
| TV Show | 2024 | An Oprah Special: Shame, Blame and the Weight Loss Revolution | Self | 2 |
| TV Show | 2019 | RuPaul | Self | 1 |
| TV Show | 2007 | Keeping Up with the Kardashians | Self | 1 |
| TV Show | 1968 | 60 Minutes | Self (archive footage) | 1 |
| TV Show | 1981 | Entertainment Tonight | Self | 6 |
| TV Show | 2022 | The Checkup with Dr. David Agus | Self | 1 |
| TV Show | 2019 | Oprah's Book Club | Self - Host | 14 |
| TV Show | 2004 | On Air with Ryan Seacrest | Self | 1 |
| TV Show | 2009 | Watch What Happens Live with Andy Cohen | Self - Guest | 1 |
| TV Show | 2018 | Patriot Act with Hasan Minhaj | Self (archive footage) | 1 |
| TV Show | 2015 | Close Up with The Hollywood Reporter | Self | 1 |
| TV Show | 1986 | The Oprah Winfrey Show | Self - Host | 1 |
| TV Show | 2011 | Super Soul Sunday | Herself - Host | 162 |
| TV Show | 2002 | Oprah After the Show | Herself - Host | 1 |
| TV Show | 2020 | Visible: Out on Television | Self | 5 |
| TV Show | 2022 | The Hair Tales | Self | 1 |
| TV Show | 2020 | Oprah Talks COVID-19 | Self - Host | 13 |
| TV Show | 2020 | Some Good News | Self | 1 |
| TV Show | 2020 | OWN Spotlight: Where Do We Go From Here? | Host | 2 |
| TV Show | 2020 | The Oprah Conversation | Self - Host | 15 |
| TV Show | 2020 | First Ladies | Self (archive footage) | 1 |
| TV Show | 1979 | CBS News Sunday Morning | Self | 1 |
| TV Show | 1998 | Parkinson | Self | 1 |
| TV Show | 2014 | Variety Studio: Actors on Actors | Self | 1 |
| TV Show | 1987 | Dolly | Self | 1 |
| TV Show | 2009 | Life | Self - Narrator, U.S. Broadcast (voice) | 10 |
| TV Show | 1953 | The Oscars | Self - Presenter | 1 |
| TV Show | 2020 | America's Great Divide: From Obama to Trump | Self (archive footage) | 2 |
