| Movie | 1997 | Face/Off | Wanda | |
| Movie | 2008 | One Missed Call | Mickey Lee | |
| Movie | 1996 | It's My Party | Charlene Lee | |
| Movie | 2003 | Richard Pryor: I Ain't Dead Yet, #*%$#@!! | Self | |
| Movie | 2004 | Margaret Cho: CHO Revolution | Herself | |
| Movie | 2016 | The girls I know... get their hands dirty. | Mom (voice) | |
| Movie | 1995 | The Doom Generation | Clerk's Wife | |
| Movie | 2009 | How Bruce Lee Changed the World | Self | |
| Movie | 2010 | I Am Comic | Self | |
| Movie | 2021 | Tara, Messenger of Death | Ms. Corrigan | |
| Movie | 1998 | The Thin Pink Line | Asia Blue / Terry | |
| Movie | 2000 | Margaret Cho: I'm the One That I Want | Herself | |
| Movie | 1997 | Fakin' Da Funk | May-Ling | |
| Movie | 2022 | I, Challenger | June | |
| Movie | 1999 | Get Bruce! | Self | |
| Movie | 1999 | The Tavern | Carol | |
| Movie | 1998 | Ground Control | Amanda | |
| Movie | 2023 | Gianna | Kimberly | |
| Movie | 1997 | Sweethearts | Noreen | |
| Movie | 2009 | Margaret Cho: Beautiful | Self | |
| Movie | 2005 | Margaret Cho: Assassin | Herself | |
| Movie | 2002 | Margaret Cho: Notorious C.H.O. | Herself | |
| Movie | 2022 | Bury Me | June | |
| Movie | 2023 | Maxine | Maxine | |
| Movie | 2011 | Miss Representation | Self | |
| Movie | 1999 | Funny Is Money | Self | |
| Movie | 1994 | Attack of the 5 Ft. 2 Women | Connie Tong | |
| Movie | 2023 | Prom Pact | Ms. Chen | |
| Movie | 2012 | See a Little Light: A Celebration of the Music and Legacy of Bob Mould | Self | |
| Movie | 2011 | Margaret Cho: Cho Dependent | Self | |
| Movie | 2013 | In Transition | Tawny Kim | |
| Movie | 2022 | Soul of a Nation Presents: Together As One: Celebrating Asian American, Native Hawaiian and Pacific Islander Heritage | Self | |
| Movie | 2013 | Wedding Palace | The Shaman | |
| Movie | 2023 | A Big Gay Hairy Hit! Where the Bears Are: The Documentary | | |
| Movie | 2005 | Bam Bam and Celeste | Celeste / Mommy | |
| Movie | 2022 | Stand Out: An LGBTQ+ Celebration | | |
| Movie | 2022 | Jane Fonda & Lily Tomlin: Ladies Night Live | Self | |
| Movie | 2022 | Queer Riot | | |
| Movie | 1997 | Pink as the Day She Was Born | | |
| Movie | 2022 | The Best of Netflix Is a Joke: The Festival | Self | |
| Movie | 2004 | Nobody Knows Anything! | Rental Car Agent | |
| Movie | 2023 | No One Asked You | Self | |
| Movie | 2015 | #BeRobin the Movie | | |
| Movie | 2024 | The Listener | Corinne | |
| Movie | 2022 | Sex with Sue | Self | |
| Movie | 2015 | Call Me Lucky | Self | |
| Movie | 2015 | Adventures in Comedy | | |
| Movie | 2017 | Curtain Down | The Mentor | |
| Movie | 2012 | Exotic World and the Burlesque Revival | (voice) | |
| Movie | 2015 | Senior Project | Ms. Ghetty | |
| Movie | 2006 | Falling for Grace | Janie | |
| Movie | 2009 | The Snake | The Expert | |
| Movie | 2006 | My Name is Jackie Beat | | |
| Movie | 2024 | Dolly Parton's Pet Gala | | |
| Movie | 2015 | Tooken | Brown Finger | |
| Movie | 1994 | Margaret Cho: HBO Comedy Half-Hour | | |
| Movie | 2007 | Girltrash! | Min Suk | |
| Movie | 2024 | All That We Love | Emma | |
| Movie | 2015 | Margaret Cho: PsyCHO | Self | |
| Movie | 2024 | Outstanding: A Comedy Revolution | Self | |
| Movie | 2015 | Do I Sound Gay? | Self | |
| Movie | 2009 | 17 Again | Mrs. Dell | |
| Movie | 1999 | Can't Stop Dancing | JoJo | |
| Movie | 2011 | Sexing the Transman | Self | |
| Movie | 2016 | Hurricane Bianca | Wig Shop Owner | |
| Movie | 2014 | Lennon or McCartney | Self | |
| Movie | 2008 | Underbelly | | |
| Movie | 2017 | Everybody Has an Andy Dick Story | | |
| Movie | 2007 | Dresden Dolls: Live at the Roundhouse London | | |
| Movie | 2025 | Queens of the Dead | Pops | |
| Movie | N/A | Untitled Mitch Hedberg documentary | Self | |
| Movie | 2024 | Cora Bora | Electra | |
| Movie | 1999 | $pent | Travel Agent | |
| Movie | 2002 | Grocery Store | Store Clerk(voice) | |
| Movie | 2024 | TURA! | Narrator (voice) | |
| Movie | 2012 | It Gets Better | Self | |
| Movie | 2017 | Sharknado 5: Global Swarming | Simone | |
| Movie | 2017 | The Untold Tales of Armistead Maupin | Self | |
| Movie | 1998 | The Rugrats Movie | Lt. Klavin (voice) | |
| Movie | 2017 | Bright | Sergeant Ching | |
| Movie | 2017 | This Is Bob Hope... | Self | |
| Movie | 2020 | Friendsgiving | Fairy Gay Mother #2 | |
| Movie | 2008 | Prop 8: The Musical | California Gays and The People That Love Them | |
| Movie | 2018 | Hocus Pocus 25th Anniversary Halloween Bash | Self | |
| Movie | 2018 | The Advocate Celebrates 50 Years: A Long Road to Freedom | Self | |
| Movie | 1998 | The N.Y. Friars Club Roast Of Drew Carey | Herself | |
| Movie | 2012 | The Immigrant | Margaret | |
| Movie | 2019 | Still Laugh-In: The Stars Celebrate | Self | |
| Movie | 2010 | Gaze | Self (archive footage) | |
| Movie | 2020 | Faith Based | Jane | |
| Movie | 2019 | Trixie Mattel: Skinny Legend | Margaret Cho | |
| Movie | 2018 | Bloodlines: The Art and Life of Vincent Castiglia | Self | |
| Movie | 2002 | Miss America | Self | |
| Movie | 1994 | HBO Comedy Half-Hour: Margaret Cho | | |
| Movie | 2020 | Feeding America Comedy Festival | Self | |
| Movie | 1994 | Angie | Admissions Nurse #2 | |
| Movie | 2017 | Katy Perry: Will You Be My Witness? | Self | |
| Movie | 2013 | Amelia's 25th | Babs | |
| Movie | 2020 | Mae West: Dirty Blonde | Self | |
| Movie | 2020 | Over the Moon | Auntie Ling / Gretch (voice) | |
| Movie | 2009 | Annul Victory | Self | |
| Movie | 2019 | Undateable John | Hilda | |
| Movie | 2017 | Alaska Is a Drag | Jan | |
| Movie | 2021 | Hysterical | Self | |
| Movie | 2021 | Koreatown Ghost Story | Mrs. Moon | |
| Movie | 2022 | Sex Appeal | Ma Deb | |
| Movie | 2006 | Phone Sex | Caller | |
| Movie | 2021 | Good on Paper | Margot | |
| Movie | 2021 | The Alaska Thunderfuck Extra Special Comedy Special | Self | |
| Movie | 2021 | Tig Notaro: Drawn | Self | |
| Movie | 2021 | The Sixth Reel | Doris | |
| Movie | 2020 | The Paley Center Salutes Law & Order: SVU | Self | |
| Movie | 2022 | Fire Island | Erin | |
| Movie | N/A | I Want Your Sex | | |
| Movie | 2016 | Ellie Kemper: Unbreakable Comedy Gala | | |
| Movie | 2025 | It's Dorothy! | Self | |
| Movie | N/A | Cheap AF | | |
| Movie | N/A | Thoughts and Prayers | Principal Linda | |
| Movie | N/A | Ron Forever | Gloria | |
| Movie | 2025 | Every Time You Lose Your Mind: A Film About Failure | Self | |
| Movie | N/A | Every Time You Lose Your Mind: A Film About Failure | Self | |
| Movie | N/A | By the Roots | Janine Sorrel | |
| TV Show | 2006 | 'Til Death | Nicole | 3 |
| TV Show | 2005 | Ghost Whisperer | Professor Avery Grant | 3 |
| TV Show | 1993 | The Nanny | Caryn | 1 |
| TV Show | 2006 | The Lost Room | Suzie Kang | 3 |
| TV Show | 1992 | The Golden Palace | Fong | 1 |
| TV Show | 2012 | Finding Your Roots | Self | 1 |
| TV Show | 2006 | 30 Rock | Kim Jong-Il | 2 |
| TV Show | 1994 | All-American Girl | Margaret Kim | 19 |
| TV Show | 1996 | C Bear and Jamal | | 16 |
| TV Show | 2015 | The Late Show with Stephen Colbert | Self | 1 |
| TV Show | 1997 | The View | Self | 1 |
| TV Show | 2020 | Tournament of Laughs | Self | 1 |
| TV Show | 2019 | A Little Late with Lilly Singh | Self | 1 |
| TV Show | 2016 | Full Frontal with Samantha Bee | Self | 1 |
| TV Show | 2005 | Wisecrack | | 14 |
| TV Show | 2019 | Good Trouble | Margaret Cho | 3 |
| TV Show | 2021 | Pride | Self | 6 |
| TV Show | 2009 | Drop Dead Diva | Teri Lee | 78 |
| TV Show | 1997 | The View | Self - Guest | 1 |
| TV Show | 2007 | Girltrash! | Min Suk | 11 |
| TV Show | 1992 | Red Shoe Diaries | Phone Sex Worker | 1 |
| TV Show | 2019 | The Masked Singer | Poodle (archive footage) | 1 |
| TV Show | 2021 | Doogie Kamealoha, M.D. | Frankie | 1 |
| TV Show | 2023 | Not Dead Yet | Miss Cassandra | 1 |
| TV Show | 2021 | The Celebrity Dating Game | Self | 1 |
| TV Show | 2023 | Frog and Toad | Turtle (voice) | 4 |
| TV Show | 2013 | Norm Macdonald Live | Self | 1 |
| TV Show | 2022 | Dr. Jackie | Margaret Cho | 1 |
| TV Show | 2021 | Call Me Kat | Val Park | 1 |
| TV Show | 2005 | Hell's Kitchen | Self - Restaurant Patron | 1 |
| TV Show | 2021 | What's My Game? | Self - Contestant | 1 |
| TV Show | 2015 | Dr. Ken | Dr. Wendi | 1 |
| TV Show | 2012 | Comedians in Cars Getting Coffee | Self | 1 |
| TV Show | 2014 | Late Night with Seth Meyers | Self | 1 |
| TV Show | 2010 | The Green Room with Paul Provenza | Self | 1 |
| TV Show | 2016 | Party Legends | | 1 |
| TV Show | 2010 | Pound Puppies | Mrs. Wattana (voice) | 1 |
| TV Show | 2020 | I Can See Your Voice | | 1 |
| TV Show | 2015 | The Mr. Peabody & Sherman Show | Mulan (voice) | 1 |
| TV Show | 1998 | Hollywood Squares | Self - Panelist | 5 |
| TV Show | 2005 | Dancing with the Stars | Self - Contestant | 6 |
| TV Show | 2012 | Gotham Comedy Live | Self - Host | 1 |
| TV Show | 2014 | Celebrity Name Game | Self | 2 |
| TV Show | 2006 | 30 Rock | Kim Jong-Un | 1 |
| TV Show | 2020 | Awkwafina Is Nora from Queens | Mistress Jupiter | 2 |
| TV Show | 2017 | Truth & Iliza | Self | 1 |
| TV Show | 2007 | Rick & Steve: The Happiest Gay Couple in All the World | Condie Ling / Jessica (voice) | 2 |
| TV Show | 2007 | Rick & Steve: The Happiest Gay Couple in All the World | Condie Ling (voice) | 6 |
| TV Show | 2007 | Rick & Steve: The Happiest Gay Couple in All the World | Condie Ling / Kathy Griffin (voice) | 1 |
| TV Show | 2016 | The $100,000 Pyramid | Self | 1 |
| TV Show | 2019 | The Kelly Clarkson Show | | 2 |
| TV Show | 2022 | Sherri | Self - Guest | 1 |
| TV Show | 1995 | Happily Ever After: Fairy Tales for Every Child | Uhmuhni (voice) | 1 |
| TV Show | 1995 | Happily Ever After: Fairy Tales for Every Child | Hul Muh Ni (voice) | 1 |
| TV Show | 2010 | America: The Story of Us | Self | 1 |
| TV Show | 2008 | Celebrity Family Feud | Self | 1 |
| TV Show | 2016 | Hollywood Medium with Tyler Henry | Self - Guest | 1 |
| TV Show | 2020 | House Hunters Comedians On Couches: Unfiltered | Self - Guest Star | 1 |
| TV Show | 1998 | Sex and the City | Lynne | 1 |
| TV Show | 2009 | RuPaul's Drag Race | | 1 |
| TV Show | 2010 | The Fran Drescher Show | | 1 |
| TV Show | 1996 | Never Mind the Buzzcocks | Self | 1 |
| TV Show | 2016 | Martha & Snoop's Potluck Dinner Party | Self | 1 |
| TV Show | 2009 | Celebrity Ghost Stories | Self | 1 |
| TV Show | 2019 | Miracle Workers | God's Mom | 1 |
| TV Show | 2016 | To Tell the Truth | Self - Panelist | 1 |
| TV Show | 2012 | The Burn with Jeff Ross | Self | 1 |
| TV Show | 2019 | The Masked Singer | Poodle | 1 |
| TV Show | 2009 | Watch What Happens Live with Andy Cohen | Self - Guest | 2 |
| TV Show | 2011 | SNL Korea | Self | 1 |
| TV Show | 2018 | This Close | Zagat | 1 |
| TV Show | 2021 | The Great North | Jan (voice) | 1 |
| TV Show | 2022 | The Wheel | Self - Expert | 1 |
| TV Show | 2019 | Lights Out with David Spade | Self - Panelist | 1 |
| TV Show | 2015 | World's Funniest Fails | Self - Panelist | 1 |
| TV Show | 2018 | Unapologetic with Aisha Tyler | Self | 1 |
| TV Show | 1999 | Law & Order: Special Victims Unit | Evelyn Lee | 1 |
| TV Show | 2015 | Metropolis | | 3 |
| TV Show | 2009 | RuPaul's Drag Race | Self - Special Guest | 1 |
| TV Show | 2020 | The Flight Attendant | Utada | 2 |
| TV Show | 2017 | The Bold Type | Margaret Cho | 1 |
| TV Show | 1999 | Family Guy | Hee-Sun (voice) | 1 |
| TV Show | 2017 | Bill Nye Saves the World | Pistachio (voice) | 1 |
| TV Show | 2009 | RuPaul's Drag Race | Self - Guest Judge | 1 |
| TV Show | 2020 | Visible: Out on Television | Self - Narrator (voice) | 5 |
| TV Show | 2003 | Real Time with Bill Maher | Self | 2 |
| TV Show | 2023 | History of the World: Part II | Sophia | 1 |
| TV Show | 2018 | 25 Words or Less | | 1 |
| TV Show | 2024 | Zombies: The Re-Animated Series | Ashley (voice) | 2 |
| TV Show | 1986 | American Masters | Self | 1 |
| TV Show | 2024 | Kite Man: Hell Yeah! | Rebecca Chen (voice) | 4 |
| TV Show | 2011 | Bubble Guppies | The Kraken (voice) | 1 |
| TV Show | 2016 | Swedish Dicks | Agent Poll | 1 |
| TV Show | 2013 | Beat Bobby Flay | Self | 1 |
| TV Show | 2024 | Doctor Odyssey | Judy Riva | 1 |
| TV Show | 2013 | The Last Leg | Self | 1 |
| TV Show | 2025 | Hollywood Squares | Self | 2 |
| TV Show | 2024 | After Midnight | Self | 1 |
| TV Show | 1998 | GayVN Awards | Self - Host | 1 |
| TV Show | 2022 | Celebrity Jeopardy! | Self | 2 |
| TV Show | 1984 | AVN Awards | Self | 1 |
| TV Show | 1995 | Dr. Katz, Professional Therapist | Margaret (voice) | 1 |
| TV Show | 1994 | The Critic | (voice) | 1 |
| TV Show | 2023 | Will Trent | Therapist | 1 |
| TV Show | 2021 | Last Meals | | 1 |
| TV Show | 1996 | Pulp Comics | herself | 1 |
| TV Show | 1989 | 1/2 Hour Comedy Hour | self | 1 |
| TV Show | 1994 | Duckman | Mai Lin (voice) | 1 |
