| Movie | 2003 | Hollywood Homicide | Olivia Robidoux | |
| Movie | 2009 | I Can Do Bad All By Myself | Wilma | |
| Movie | 1980 | The Sensational Shocking Wonderful Wacky 70's | Self | |
| Movie | 2007 | Classic Soul at the BBC | Self (archive footage) | |
| Movie | 1995 | The History of Rock 'n' Roll | Self | |
| Movie | 2004 | Original Up-Town Divas | | |
| Movie | 2003 | Ladies Night - Most Famous Hits | | |
| Movie | 1978 | Rock 'n' Roll Sports Classic | | |
| Movie | 1976 | Pipe Dreams | Maria Wilson | |
| Movie | 1986 | Sisters In The Name of Love | Self | |
| Movie | 1977 | Rolling Stone Magazine: The 10th Anniversary | Self - Performer | |
| Movie | 2022 | I'm Glad It's Christmas | Cora Ross | |
| Movie | 1990 | Night of 100 Stars III | Self | |
| Movie | 2014 | Seasons of Love | Ms. Angie | |
| Movie | 2006 | Chris Botti Live: With Orchestra and Special Guests | | |
| Movie | 2015 | Queens of Soul at the BBC | Self (archive footage) | |
| Movie | 1987 | Desperado | Mona Lisa | |
| Movie | 2006 | Holidaze: The Christmas That Almost Didn't Happen | Candie (voice) | |
| Movie | 2001 | Tom Jones | Duets by Invitation Only | Self | |
| Movie | 2006 | Unbeatable Harold | Phyllis | |
| Movie | 2010 | …Sings Neil Diamond | Self (archive footage) | |
| Movie | N/A | Mirroring Michael Jackson | Self | |
| Movie | 2004 | VH1 Divas 2004 | Self | |
| Movie | 2016 | Almost Christmas | Dorothy | |
| Movie | 2024 | A Little Women's Christmas | Bertie Henderson | |
| Movie | 1973 | Save the Children | Self | |
| Movie | 1969 | Black Woodstock | | |
| Movie | 1988 | Sesame Street | Put Down the Duckie: An All-Star Musical Special | Self | |
| Movie | 2019 | The Apollo | Self (archive footage) | |
| Movie | 2018 | Q85: A Musical Celebration for Quincy Jones | Self | |
| Movie | 2001 | Michael Jackson: 30th Anniversary Celebration | Self | |
| Movie | 1973 | The Midnight Special Legendary Performances 1973 | Self | |
| Movie | 1974 | The Midnight Special Legendary Performances 1974 | Self | |
| Movie | 2018 | Mr. SOUL! | Self (archive footage) | |
| Movie | 2021 | Coming 2 America | Gladys Knight | |
| Movie | 2021 | Dionne Warwick: Don't Make Me Over | Self | |
| Movie | 1993 | Twenty Bucks | Mrs. McCormac | |
| Movie | 1986 | Women in Rock | Self | |
| Movie | 1993 | Michael Jackson Talks... to Oprah | Self | |
| Movie | 2024 | American Music Awards 50th Anniversary Special | Self | |
| Movie | 2024 | A Motown Christmas | Self | |
| Movie | 2004 | AFI's 100 Years... 100 Songs: America's Greatest Music in the Movies | Self | |
| Movie | 1996 | Atlanta 1996: Games of the XXVI Olympiad - Opening Ceremony | Self | |
| Movie | 1988 | WrestleMania IV | Self | |
| Movie | 2023 | Sunday Best | Self (archive footage) | |
| TV Show | 1964 | Top of the Pops | Self | 1 |
| TV Show | 2005 | The Situation Room with Wolf Blitzer | | 1 |
| TV Show | 1996 | The Jamie Foxx Show | Janice King | 2 |
| TV Show | 2003 | Las Vegas | Gladys Knight | 1 |
| TV Show | 1975 | The Jeffersons | | 1 |
| TV Show | 1997 | The View | Self | 1 |
| TV Show | 2006 | Celebrity Duets | | 9 |
| TV Show | 1994 | New York Undercover | Natalie | 2 |
| TV Show | 2002 | American Idol | Self - Guest Judge | 1 |
| TV Show | 2006 | 30 Rock | Gladys Knight | 1 |
| TV Show | 2020 | VERZUZ | Self | 1 |
| TV Show | 1989 | Baywatch | | 1 |
| TV Show | 1976 | The Muppet Show | Self - Special Guest Star | 1 |
| TV Show | 1997 | Ally McBeal | Gladys Knight | 1 |
| TV Show | 1985 | Charlie & Co. | | 18 |
| TV Show | 1992 | Out All Night | Self | 1 |
| TV Show | 2003 | American Juniors | | 17 |
| TV Show | 2019 | The Masked Singer | Queen Bee (archive footage) | 1 |
| TV Show | 2019 | The Masked Singer | Self - Video Message | 1 |
| TV Show | 1974 | American Music Awards | Self - Musical Guest | 1 |
| TV Show | 1972 | The Midnight Special | Self | 2 |
| TV Show | 1972 | The Midnight Special | Host | 2 |
| TV Show | 2010 | Hawaii Five-0 | Lou's mother | 1 |
| TV Show | 1993 | Intimate Portrait | Self | 1 |
| TV Show | 2002 | American Idol | Self | 1 |
| TV Show | 2005 | Dancing with the Stars | Self - Contestant | 11 |
| TV Show | 1962 | The Tonight Show Starring Johnny Carson | Self | 3 |
| TV Show | 2016 | Star | Gladys Knight | 2 |
| TV Show | 1972 | The Midnight Special | Self (as Gladys Knight & The Pips) | 1 |
| TV Show | 1987 | A Different World | Herself | 1 |
| TV Show | 1995 | Happily Ever After: Fairy Tales for Every Child | Chocolate (voice) | 1 |
| TV Show | 2019 | The Masked Singer | Bee | 1 |
| TV Show | 1998 | VH1 Divas | Self -Performer | 1 |
| TV Show | 1978 | The Kennedy Center Honors | Self - Honoree | 1 |
| TV Show | 2012 | The First Family | Grandma Carolyn | 9 |
| TV Show | 1968 | The Dick Cavett Show | Self - Guest | 1 |
| TV Show | 2010 | Hot in Cleveland | Miss Shonda | 1 |
| TV Show | 1961 | The Mike Douglas Show | Self | 1 |
| TV Show | 1971 | Soul Train | | 1 |
| TV Show | 2020 | By Whatever Means Necessary: The Times of Godfather of Harlem | Herself | 4 |
| TV Show | 1962 | The Merv Griffin Show | Self | 1 |
