Born in a cemetery, Kitaro is the last survivor of the ghost tribe. Raised by a human father, Mizuki, and accompanied by his biological father reincarnated as an eyeball, Medama Oyaji, the young Kitaro will have to learn to live in this world governed by science where yokai no longer have a place in popular belief.
A patient at a hospital has become a ghost. Mizuki, a company employee, is entrusted with the task of investigating the cause. His investigation leads him to a couple who claim to be the "last survivors of the Ghost tribe".
The grave of Yasha, who had been sleeping for a long time, has been uncovered. At the same time, the vampire Dracula came to Japan. Yasha and the vampire compete for one prey. The victim is the president of a certain company, the person who ordered Mizuki to investigate the cause of the ghost patient. Because of Mizuki's disappearance, the president's company is on the verge of bankruptcy.
Trump Omoi, a singer who meets Mizuki by chance, learns of the existence of a sinister child named Kitaro. One night, Nezumi-otoko plants the seeds of a vampire tree in Omoi. Omoi's frightened body gradually turns into a tree.
Neko is a girl Kitaro is in love with. Neko dreams of becoming a singer. Kitaro will do his best to make it happen. With the support of Trump Omoi, she quickly becomes a celebrity. However, someone releases a rat onto her stage.
Kitaro's first love, Neko, is dead. Kitaro is devastated and tries to go to Hell to see her, but someone steals his chanchanko. The culprit is the fake Kitaro. The fake Kitaro walks around Hell with the chanchanko and plots with Nezumi-Otoko to obtain a large sum of money. Kitaro's father, Medama-Oyaji goes to hell and tries to bring Neko back.